NLRP3抗体
anti-NLRP3/NALP3, mAb
- 产品特性
- 相关资料
- Q&A
- 参考文献
NLRP3抗体
◆产品描述
炎性体是介导caspase-1活化的多蛋白复合物, 它可以促进除了别的以外的促炎细胞因子白介素（IL）-1β和 IL-18的分泌物。nod样受体家族的 NLRP3/NALP3是发危险信号到caspase-1活化炎性体的关键组成部分。NLRP3缺陷是导致家族性寒冷型自身炎症综合征1型（FCAS1）、Muckle-Wells 综合征 (MWS) 和慢性炎症性神经，皮肤，关节综合征的原因。
◆规格 & 操作
| 产品数据 | ||
| 产品别名 | NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein |
|
| 抗小鼠NLRP3/NALP3单抗（Cryo-1） | NLRP3/NALP3单抗（Cryo-2） | |
| 特性 | ||
| 集落 | Cryo-1 | Cryo-2 |
| 同型 | 小鼠IgG2b | |
| 来源/宿主 | 纯化浓缩的杂交瘤细胞组织培养上清液。 | |
| 应用 | Western印迹：(1 μg/mL) | Western印迹：(1 μg/mL) 免疫细胞化学：1. see J. Virol. 85, 13019 (2011) 2. Nat. Immunol. 13, 255 (2012) 免疫沉淀：in RIPA buffer, see Mol. Cell 49, 331 (2012) 免疫组化：1:200, for a protocol see Nature 493, 67 (2012) ChIP assay：2µg/DNA of 10E6 cells (see Nat. Immunol. 16, 859 (2015)) |
| 交叉反应性 | 小鼠 | 人，小鼠 |
| 特异性 | 识别小鼠NLRP3/ NALP3 | 识别人，小鼠的NLRP3/ NALP3 |
| 纯度 | ≥95% (SDS-PAGE) | |
| 浓度详情 | Protein G-affinity purified. | |
| 浓度 | 1 mg/mL | |
| 配方 | 液体、含10％甘油的PBS和0.02％的叠氮化钠。 | |
| 产品类型 | 单克隆抗体 | |
| 运输和装卸 | ||
| 运输 | BLUE ICE | |
| 短期储存温度 | +4°C | |
| 长期储存温度 | -20°C | |
| 建议处置方式 | 开封后, 分装并储存在-20°C的地方，避免冷冻/解冻循环。 | |
| 使用/稳定性 | 收货后保存在-20°C的地方，可稳定保存至少一年。 | |
◆案例应用
抗小鼠NLRP3/NALP3单抗（Cryo-1）
使用抗小鼠NLRP3/NALP3单抗（Cryo-1）检测小鼠巨噬细胞中NLRP3的含量。
细胞提取物来源于小鼠巨噬细胞WT（泳道1）或NLRP3 KO（泳道2）, 在还原条件下用SDS-PAGE 分离, 转移到硝酸纤维素膜后，用抗小鼠NLRP3/NALP3单抗（Cryo-1） (1μg/ml) 孵育并检测。在化学发光检测系统中，蛋白质是可视化的。
NLRP3/NALP3单抗（Cryo-2)
用anti-NLRP3/NALP3, mAb (Cryo-2)分别检测人和小鼠的THP1 细胞及巨噬细胞中的NLRP3/NALP3 含量。
A）细胞提取物来自于人THP1 细胞（泳道1）或THP1 表达的shRNA hNLRP3（泳道2）
B）细胞提取物来自于小鼠巨噬细胞 WT（泳道1）或NLRP3/NALP3 KO（泳道2），用SDS-PAGE 电泳分离，转转移到硝酸纤维素膜后，用anti-NLRP3/NALP3, mAb (Cryo-2) (1 μg/ml) 孵育，并检测。在化学发光检测系统中，蛋白质是可视化的。
使用NLRP3（Cryo-2）单抗（产品编号AG-20B-0014）在小鼠巨噬细胞中检测小鼠NLRP3。
实验方法：从小鼠巨噬细胞（BMDM）WT + / +（lane 1），NLRP3 +/-（lane 2）或NLRP3 – / – （lane 3）的
细胞提取物，有或没有用LPS（50ng / mL）处理3 h，在还原条件下通过SDS-PAGE分离，转移至硝酸
纤维素膜并与NLRP3（Cryo-2）的mAb（1μg/ mL）共同孵育。 蛋白通过化学发光显影。图片由德国
慕尼黑大学的Dr. Olaf Gross提供。
◆产品列表
|
产品编号 |
产品名称 |
规格 |
|
AG-20B-0006-C100 |
anti-NLRP3/NALP3 (mouse), mAb (Cryo-1) |
100 ug |
|
AG-20B-0014-C100 |
anti-NLRP3/NALP3, mAb (Cryo-2) |
100 ug |
欲了解更多产品相关资讯，请点击文字：
AG NLRP3 Inflammasome
AG免疫&炎症研究
NLRP3/NALP3单抗（Cryo-2)的参考文献
|
1. |
Thioredoxin-interacting protein links oxidative stress to inflammasome activation: R. Zhou, et al.; Nature Immunology 11, 136 (2010) |
|
2. |
The NLRP3 inflammasome promotes renal inflammation and contributes to CKD: A. Vilaysane, et al.; J. Am. Soc. Nephrol. 21, 1732 (2010) |
|
3. |
A role for mitochondria in NLRP3 inflammasome activation: R. Zhou, et al.; Nature 469, 221 (2011) |
|
4. |
Differential expression of NLRP3 among hematopoietic cells: G. Guarda, et al.; J. Immunol. 186, 2529 (2011) |
|
5. |
Mechanism of Impaired NLRP3 Inflammasome Priming by Monophosphoryl Lipid A: C.A. Embry, et al.; Sci. Signal. 4, ra28 (2011) |
|
6. |
Measles Virus V Protein Inhibits NLRP3 Inflammasome-Mediated Interleukin-1 Secretion: N. Komune, et al.; J. Virol. 85, 13019 (2011) |
|
7. |
Novel role of PKR in inflammasome activation and HMGB1 release: B. Lu, et al.; Nature 488, 670 (2012) |
|
8. |
Deubiquitination of NLRP3 by BRCC3 Critically Regulates Inflammasome Activity: B.F. Py, et al.; Mol. Cell 49,331 (2012) |
|
9. |
NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 67 (2012) |
|
10. |
Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012) |
|
11. |
Inflammasome-independent NLRP3 augments TGF-β signaling in kidney epithelium: W. Wang, et al.; J. Immunol. 190, 1239 (2013) |
|
12. |
Omega-3 Fatty Acids Prevent Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome Activation: Y. Yan, et al.; Immunity 38, 1154 (2013) |
|
13. |
LRRFIP2 negatively regulates NLRP3 inflammasome activation in macrophages by promoting Flightless-I-mediated caspase-1 inhibition: J. Jin, et al.; Nat. Commun. 4, 2075 (2013) |
|
14. |
Proapoptotic Chemotherapeutic Drugs Induce Noncanonical Processing and Release of IL-1β via Caspase-8 in Dendritic Cells: C. Antonopoulus, et al. J. Immunol. 191, 4789 (2013) |
|
15. |
Constitutively activated NLRP3 inflammasome causes inflammation and abnormal skeletal development in mice: S.L. Bonar, et al.; PLoS One 7, e35979 (2012) |
|
16. |
FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes: P. Gurung, et al.; J. Immunol. 192, 1835 (2014) |
|
17. |
XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep.7, 1796 (2014) |
|
18. |
The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. 15, 727 (2014) |
|
19. |
The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014) |
|
20. |
Toward a chemical marker for inflammatory disease: a fluorescent probe for membrane-localized thioredoxin: M.H. Lee, et al.; JACS 136, 8430 (2014) |
|
21. |
Prostaglandin E2 inhibits NLRP3 inflammasome activation through EP4 receptor and intracellular cyclic AMP in human macrophages: M. Sokolowska, et al.; J. Immunol. 194, 5472 (2015) |
|
22. |
The receptor NLRP3 is a transcriptional regulator of TH2 differentiation: M. Bruchard, et al.; Nat. Immunol. 16,859 (2015) |
|
23. |
NLRP3 Inflammasome Is Expressed and Functional in Mouse Brain Microglia but Not in Astrocytes: A. Gustin, et al.; PLos One 10, e0130624 (2015) |
|
24. |
Nlrp6 promotes recovery after peripheral nerve injury independently of inflammasomes: E. Ydens, et al.; J. Neuroinfl. 12, 143 (2015) |
|
25. |
The Crohn’s disease risk factor IRGM limits NLRP3 inflammasome activation by impeding its assembly and by mediating its selective autophagy: S. Mehto, et al.; Mol. Cell 73, 429 (2019) |
|
26. |
Lack of Protein Prenylation Promotes Nlrp3 Inflammasome Assembly in Human Monocytes: O.P. Skinner, et al.; J. Allergy Clin. Immunol. (Epub ahead of print) (2019) |
| 产品编号 | 产品名称 | 产品规格 | 产品等级 | 产品价格 |
| AG-20B-0006-C100 | anti-NLRP3/NALP3 (mouse), mAb (Cryo-1) 抗小鼠NLRP3/NALP3单抗（Cryo-1） |
100 ug | – | – |
| AG-20B-0014-C100 | anti-NLRP3/NALP3, mAb (Cryo-2) NLRP3/NALP3单抗（Cryo-2) |
100 ug | – | – |