产品数据

　产品别名

　NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin;    　Cold Autoinflammatory Syndrome 1 Protein

　抗小鼠NLRP3/NALP3单抗

　（Cryo-1）

　NLRP3/NALP3单抗

　（Cryo-2)

　特性

　集落

　Cryo-1

　Cryo-2

　同型

　小鼠IgG2b

　来源/宿主

　纯化浓缩的杂交瘤细胞组织培养上清液。

　免疫原/抗原

　重组小鼠 NLRP3/NALP3 (pyrin domain/aa 1-93).

　应用

　Western印迹: (1μg/ml)

　Western印迹: (1μg/ml)
    免疫细胞化学：(see J. Virol. 85, 13019 (2011) and Nat. Immunol. 13, 255    (2012))
    免疫沉淀: (in RIPA buffer, see    Mol. Cell 49, 331 (2012))
    免疫组化: (1:200, for a protocol    see Nature 493, 67 (2012))
    ChIP assay: 2µg/DNA of 10E6 cells (see Nat. Immunol. 16, 859    (2015))

　交叉反应性

　小鼠                    

　人，小鼠

　特异性

　识别小鼠NLRP3/ NALP3

　识别人，小鼠的NLRP3/ NALP3

　纯度

　≥95% (SDS-PAGE)

　浓度详情

　Protein G-affinity purified.

　浓度

　1mg/ml

　配方

　液体、含10％甘油的PBS和0.02％的叠氮化钠。

　产品类型

　单克隆抗体

　运输和装卸

　运输

　BLUE ICE

　短期储存温度

　+4°C

　长期储存温度

　-20°C

　建议处置方式

　开封后, 分装并储存在-20°C的地方，避免冷冻/解冻循环。

　使用/稳定性

　收货后保存在-20°C的地方，可稳定保存至少一年。

产品编号

产品名称

规格

AG-20B-0006-C100

anti-NLRP3/NALP3    (mouse), mAb (Cryo-1)
     抗小鼠NLRP3/NALP3单抗（Cryo-1）

100 ug

AG-20B-0014-C100

anti-NLRP3/NALP3,    mAb (Cryo-2)
     NLRP3/NALP3单抗（Cryo-2)

100 ug

1. Thioredoxin-interacting protein    links oxidative stress to inflammasome activation: R. Zhou, et al.; Nature    Immunology 11, 136 (2010)

2. The NLRP3 inflammasome promotes    renal inflammation and contributes to CKD: A. Vilaysane, et al.; J. Am. Soc.    Nephrol. 21, 1732 (2010)

3. A role for mitochondria in    NLRP3 inflammasome activation: R. Zhou, et al.; Nature 469, 221 (2011)

4. Differential expression of    NLRP3 among hematopoietic cells: G. Guarda, et al.; J. Immunol. 186, 2529 (2011)

5. Mechanism of Impaired NLRP3    Inflammasome Priming by Monophosphoryl Lipid A: C.A. Embry, et al.; Sci.    Signal. 4, ra28 (2011)

6. Measles Virus V Protein    Inhibits NLRP3 Inflammasome-Mediated Interleukin-1 Secretion: N. Komune, et    al.; J. Virol. 85, 13019 (2011)

7. Novel role of PKR in    inflammasome activation and HMGB1 release: B. Lu, et al.; Nature 488, 670 (2012)

8. Deubiquitination of NLRP3 by    BRCC3 Critically Regulates Inflammasome Activity: B.F. Py, et al.; Mol. Cell 49,331 (2012)

9. NLRP3 is activated in    Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T.    Heneka, et al.; Nature 493, 67 (2012)

10. Activation of autophagy by    inflammatory signals limits IL-1b production by targeting ubiquitinated    inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012)

11. Inflammasome-independent NLRP3    augments TGF-β signaling in kidney epithelium: W. Wang, et al.; J. Immunol. 190, 1239 (2013)

12. Omega-3 Fatty Acids Prevent    Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome    Activation: Y. Yan, et al.; Immunity 38, 1154 (2013)

13. LRRFIP2 negatively regulates    NLRP3 inflammasome activation in macrophages by promoting    Flightless-I-mediated caspase-1 inhibition: J. Jin, et al.; Nat. Commun. 4, 2075 (2013)

14. Proapoptotic Chemotherapeutic    Drugs Induce Noncanonical Processing and Release of IL-1β via Caspase-8 in    Dendritic Cells: C. Antonopoulus, et al. J. Immunol. 191, 4789 (2013)

15. Constitutively activated NLRP3    inflammasome causes inflammation and abnormal skeletal development in mice:    S.L. Bonar, et al.; PLoS One 7, e35979 (2012)

16. FADD and caspase-8 mediate    priming and activation of the canonical and noncanonical Nlrp3 inflammasomes:    P. Gurung, et al.; J. Immunol. 192, 1835 (2014)

17. XIAP Restricts TNF- and    RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell    Rep.7, 1796 (2014)

18. The adaptor ASC has    extracellular and 'prionoid' activities that propagate inflammation: B.S.    Franklin, et al.; Nat. Immunol. 15, 727 (2014)

19. The NLRP3 inflammasome is    released as a particulate danger signal that amplifies the inflammatory    response: A. Baroja-Mazo, et al.; Nat. Immunol. 15, 738 (2014)

20. Toward a chemical marker for    inflammatory disease: a fluorescent probe for membrane-localized thioredoxin:    M.H. Lee, et al.; JACS 136, 8430 (2014)

21. Prostaglandin E2 inhibits NLRP3    inflammasome activation through EP4 receptor and intracellular cyclic AMP in human    macrophages: M. Sokolowska, et al.; J. Immunol. 194, 5472 (2015)

22. The receptor NLRP3 is a    transcriptional regulator of TH2 differentiation: M. Bruchard, et al.; Nat.    Immunol. 16,859 (2015)

23. NLRP3 Inflammasome Is Expressed    and Functional in Mouse Brain Microglia but Not in Astrocytes: A. Gustin, et    al.; PLos One 10, e0130624 (2015)

24. Nlrp6 promotes recovery after    peripheral nerve injury independently of inflammasomes: E. Ydens, et al.; J.    Neuroinfl. 12, 143 (2015)