QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
- 产品型号： 12381
- 简单描述
- QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒EndoFree Plasmid Kits基于阴离子交换技术，可快速制备不含内毒素的质粒DNA。QIAfilter Cartridges过滤快速澄清裂解液。制备的DNA纯度超过两次CsCl梯度离心法所得DNA的纯度，适合超转染级纯的应用。EndoFree Plasmid Buffer Set可用于制备10次m
详细介绍
QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
纯化得到至多10 mg的无内毒素高转染级纯质粒或柯斯质粒DNA
- 纯化得到的DNA内毒素水平低于0.1 EU/µg
- 纯化流程快，同时去除内毒素
- 获得高产量、高拷贝质粒DNA
- 使用LyseBlue，获得的裂解和高产量的DNA
QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
质粒纯化方法与转染效率。
使用两种方法独立制备pRSVcat，分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中，及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit（100％）制备的DNA的转染效率表示。
质粒纯化方法与转染效率。
使用两种方法独立制备pRSVcat，分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中，及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit（100％）制备的DNA的转染效率表示。
相关产品订购信息：
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